EVTT

ExCT - Institute of Experimental and Clinical Cell Therapy

Director: Univ.-Prof. Dr. Dirk Strunk

 

 

Induced pluripotent stem cell (iPSC) as a tool for the production of therapeutically active extracellular vesicles (EVs) in regenerative medicine

 

Stem cells secrete paracrine factors including EVs that are important in cellular communication and can support the regeneration of injured tissues. Reduced oxygen conditions (hypoxia) are crucial for proliferation and self-renewal of stem cells. As hypoxia is a key regulator in development / regeneration it may also be an important factor influencing cellular communication via EVs. Therefore, we will investigate whether hypoxic pre-conditioning can impact iPSC-EV quantity and/or quality (phenotype and cargo) & thus have an impact for EV-based therapy. In a second series of experiments we will compare iPSC-derived EVs with that of their differentiated progeny to determine the impact of cell lineage specification on EV number, phenotype, cargo and function. [see homepage]

  • establishment of iPSC culture with different hypoxia conditions
  • optimize conditions for iPSC-EV production
  • large scale iPSC-EV GMP-compatible production
Dirk Strunk

Univ.-Prof. Dr. Dirk Strunk

+43 662 2420 80730 dirk.strunk@pmu.ac.at

EV-TT Member of the PMU Consortium
Paracelsus Medical University - Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS)
Transfer Center for Extracellular Vesicles - Theralytic Technologies

André Cronemberger-Andrade

Dr. André Cronemberger-Andrade

+43 662 2420 80734 andre.cronemberger@pmu.ac.at

EV-TT Scientific Project Employee
Paracelsus Medical University - Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS)
Transfer Center for Extracellular Vesicles - Theralytic Technologies